User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/09/27: Difference between revisions
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==Objective== | ==Objective== | ||
Three objectives were part of today's experiment. First, AuNP nanoparticle synthesis was repeated with constant heat. Second, bacteria was cultured to express inteins via protein expression. Lastly, DNA coding for mutant GFP (mutated on 09/20/11) was transformed. | |||
==Procedure== | ==Procedure== | ||
Revision as of 12:19, 30 November 2011
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ObjectiveThree objectives were part of today's experiment. First, AuNP nanoparticle synthesis was repeated with constant heat. Second, bacteria was cultured to express inteins via protein expression. Lastly, DNA coding for mutant GFP (mutated on 09/20/11) was transformed. ProcedureProtocol from [1], was followed in the same manner with one exception: Each cuvette was kept internally in the UV Vis at around 70 degrees Celsius. ResultsWater as a Buffer:
 Each time interval had a positive effect on the absorbance of the cuvette solution. This suggests that AUNPs were being formed as time increased. A slight disturbance during t=150 occurred such that values from wavelengths 200-300 were removed from the data set and marked as invalid.  The positive linear relationship suggests that AUNP were formed in the solution as time increased. 
 As shown above, the absorbance increased as time increased.  The positive linear relationship suggests that AUNP were formed in the solution as time increased. Notes | |
