User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/09/27: Difference between revisions
Laura Flynn (talk | contribs) (→Notes) |
Laura Flynn (talk | contribs) |
||
Line 50: | Line 50: | ||
==Results== | ==Results== | ||
Revision as of 12:26, 30 November 2011
Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html> | |
ObjectiveThree objectives were part of today's experiment. First, AuNP nanoparticle synthesis was repeated with constant heat. Second, bacteria was cultured to express inteins via protein expression. Lastly, DNA coding for mutant GFP (mutated on 09/20/11) was transformed. ProcedureAUNP synthesis: Protocol from [1], was followed in the same manner with one exception: Each cuvette was kept internally in the UV Vis at around 70 degrees Celsius. Protein Expression: Starter Culture Media, Protein Expression
DNA Transformation
ResultsAcetate as a Buffer:
As shown above, the absorbance increased as time increased. The positive linear relationship suggests that AUNP were formed in the solution as time increased. NotesDNA transformation did not appear successful, as no cells grew. |