User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/10/05: Difference between revisions
Laura Flynn (talk | contribs) |
Laura Flynn (talk | contribs) No edit summary |
||
Line 13: | Line 13: | ||
The purpose of today's experiment was to use standard concentrations Ru(bpy)(phen) to determine the sensitivity of a fluorimeter. This was then used to determine the concentration of a stock solution of maltose binding protein (MBP). | The purpose of today's experiment was to use standard concentrations Ru(bpy)(phen) to determine the sensitivity of a fluorimeter. This was then used to determine the concentration of a stock solution of maltose binding protein (MBP). | ||
The cells cultured on 09/27/11 also underwent DNA purification as well. | |||
==Procedure== | ==Procedure== | ||
MBP Concentration Determination: | |||
*5 varying concentrations of MBP were prepared using TRIS as the solvent. The 5 solutions each were composed of: | *5 varying concentrations of MBP were prepared using TRIS as the solvent. The 5 solutions each were composed of: | ||
Line 25: | Line 28: | ||
<center>[[Image:EpsilonZoom22.jpg]]</center> | <center>[[Image:EpsilonZoom22.jpg]]</center> | ||
*A graph of intensity/concentration of MBP vs. wavelength for each solution was created and used for analysis. | *A graph of intensity/concentration of MBP vs. wavelength for each solution was created and used for analysis. | ||
DNA Purification: | |||
*The DNA purification protocol was adapted from the Wizard Plus SV Minpreps DNA Purification System vacuum protocol found here: | |||
http://www.promega.com/~/media/Files/Resources/ProtCards/Wizard%20Plus%20SV%20Minipreps%20DNA%20Purification%20System%20Quick%20Protocol.ashx | |||
==Results== | ==Results== |
Revision as of 12:35, 30 November 2011
Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html> | |
ObjectiveThe purpose of today's experiment was to use standard concentrations Ru(bpy)(phen) to determine the sensitivity of a fluorimeter. This was then used to determine the concentration of a stock solution of maltose binding protein (MBP). The cells cultured on 09/27/11 also underwent DNA purification as well. ProcedureMBP Concentration Determination:
So that each following stock solution had a dilution of MBP by a factor of 10.
DNA Purification:
ResultsThe final concentration of MBP in each of the stock solutions was determined to be: This was determined using the values from the UV Vis analysis. The resulting absorbance vs. wavelength graph yielded: Each solution (A-E) was graphed as Intensity/Concentration vs. Wavelength.
The resulting graphs of both Solution A and Solution B show that the concentration of the MBP was too high to be detected on the fluorimeter. Each of these graphs had a maximum that was determined to be:
This graph shows the collective data of every group participating in this experiment. This graph will be updated as more groups record their information. Group 3, which is denoted by asterisks, is the group that this notebook's information is from. Notes
|