User:Laura Flynn/Notebook/Experimental Biological Chemistry/2011/12/07: Difference between revisions

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==Objective==
==Objective==


==Procedure==
To try to control the aggregation process of AuNP/BSA.


==Results==
==Protocol==
 
# Make 4 solutions of AuNP:
#* Solutions 1 and 2 (Au/BSA mole ratio = 35)
#*# Combine 0.5 mL of 2.5 mM HAuCl<sub>4</sub> and 0.0706 mL of 0.0177 mM BSA in a test tube.
#*# Add 5 mL of water.
#*# Place in oven at 70°C.
#* Solutions 3 and 4 (Au/BSA mole ratio = 100)
#*# Combine 0.5 mL of 2.5 mM HAuCl<sub>4</sub> and 2.02 mL of 0.0177 mM BSA in a test tube.
#*# Add 5 mL of water.
#*# Place in oven at 70°C.
# Leave solutions 1 and 3 in oven for 3.5 hr.
# Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.
 
==Data==
 
Solution 1: Au/BSA = 35, left in the oven
 
<center>[[Image:Photo3A.JPG|300px]]</center>
 
 
Solution 2: Au/BSA = 35, taken out of the oven every 30 minutes
 
<center>[[Image:Photo2A.JPG|300px]]</center>
 
 
Solution 3: Au/BSA = 100, left in the oven
 
<center>[[Image:Photo4A.JPG|300px]]</center>
 
 
Solution 4: Au/BSA = 100, taken out of the oven every 30 minutes
 
<center>[[Image:Photo1A.JPG|300px]]</center>


==Notes==
==Notes==
Solution 1 began to turn purple after 2 hr.




Revision as of 20:46, 9 December 2011

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Objective

To try to control the aggregation process of AuNP/BSA.

Protocol

  1. Make 4 solutions of AuNP:
    • Solutions 1 and 2 (Au/BSA mole ratio = 35)
      1. Combine 0.5 mL of 2.5 mM HAuCl4 and 0.0706 mL of 0.0177 mM BSA in a test tube.
      2. Add 5 mL of water.
      3. Place in oven at 70°C.
    • Solutions 3 and 4 (Au/BSA mole ratio = 100)
      1. Combine 0.5 mL of 2.5 mM HAuCl4 and 2.02 mL of 0.0177 mM BSA in a test tube.
      2. Add 5 mL of water.
      3. Place in oven at 70°C.
  2. Leave solutions 1 and 3 in oven for 3.5 hr.
  3. Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.

Data

Solution 1: Au/BSA = 35, left in the oven


Solution 2: Au/BSA = 35, taken out of the oven every 30 minutes


Solution 3: Au/BSA = 100, left in the oven


Solution 4: Au/BSA = 100, taken out of the oven every 30 minutes

Notes

Solution 1 began to turn purple after 2 hr.



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