User:Linh N Le/Notebook/2009/06/01

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(Microtubules)
Current revision (14:02, 29 September 2009) (view source)
 
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==First Enty==
==First Enty==
*Koch had mentioned that I become more "science 2.0" and so I brought it upon myself to start a notebook here on the public wiki
*Koch had mentioned that I become more "science 2.0" and so I brought it upon myself to start a notebook here on the public wiki
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==Microtubules==
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==[[/Microtubules]]==
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*We made MTs today
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*First we took an aliquot (2ul) of FITC tubulin and warmed it in the PCR
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**This caused the tubulin to polymerize into microtubules
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**We also added a buffer, 198 ul of room temperature BRB80T, to stabilize them
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*We then imaged them in the microscope
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**We saw 100's of tubes (although they are smaller than what Koch made last week http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/05/26/FITC_Microtubules_with_antifade)
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*We then made a batch with Antifade
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**The old antifade from last week was no good, but a fresh batch came out great!
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