User:Linh N Le/Notebook/2009/06/11: Difference between revisions

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*time: 4:12pm, 67% done with reformat
*time: 4:12pm, 67% done with reformat
**Still have to install windows :(
**Still have to install windows :(
*computer done installing at 5pm
*nothing comes up when i log in as the admin
**The problem i think is that the install disc that koch made does not have all the drivers that this computer needs
***I think this b/c although it is hooked up to the internet, i get no connection or response from the ethernet cable.
*Will try the drivers cd 2morrow or dl a fresh batch and send them over via usb stick


==Acetic Acid==
==Acetic Acid==

Revision as of 16:20, 11 June 2009

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New Computer

  • We finally got in our new computer for the lab
    • A brand new Dell optiplex 960
  • I, stupidly, bought the wrong video card for it (just got the integrated stock one) and that cannot handle dual screen
    • Will need to get one that can, wont be more that $100
  • I set it up and it runs just fine
  • We wiped it and are setting up the custom xp that caleb made for us that has all the networking stuff built in
  • Currently reformatting the HUGE drives, 1hr in and its at 16%
  • I hope the RAID 1 system is easy to get back in order
  • time: 4:12pm, 67% done with reformat
    • Still have to install windows :(
  • computer done installing at 5pm
  • nothing comes up when i log in as the admin
    • The problem i think is that the install disc that koch made does not have all the drivers that this computer needs
      • I think this b/c although it is hooked up to the internet, i get no connection or response from the ethernet cable.
  • Will try the drivers cd 2morrow or dl a fresh batch and send them over via usb stick

Acetic Acid

  • while waiting on the computer, i will help brigette make MT's
  • she is mixing up the acetic acid
  • Note ok MT's: I will be using the BRB80T that Igor and I made earlier this week (monday?)
    • it has been in the fridge all this time, so i think its still good
    • mixed it up using the vortexer (koch mentioned that taxol can come out of soln if it sets) and am leaving it on the counter for ~20mins to warm up before adding it to the MT's
  • Eventually i will remember the recipe for making MT's but for now I had to look it up
    • As of this monday, its 2ul of tubulin and 198ul of BRB80T (premixed)
  • Tubulin + brb80t mixed up
    • Andy is messing with the microscope right now...he has the translator stage off, so I wont bother trying to look at the MT's until he gets back and gives me the ok
  • looked at the MT's, no antifade. They are there. They fade in ~.25s
  • Now to add acetic acid to the MT's
    • Brigette made .273M Acetic Acid soln (w/DI H20)
    • She wants to add 3ul of this soln to the ~200ul of tublules (technically only 195 since i took 5ul to look under the scope)
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