New Computer
- We finally got in our new computer for the lab
- A brand new Dell optiplex 960
- I, stupidly, bought the wrong video card for it (just got the integrated stock one) and that cannot handle dual screen
- Will need to get one that can, wont be more that $100
- I set it up and it runs just fine
- We wiped it and are setting up the custom xp that caleb made for us that has all the networking stuff built in
- Currently reformatting the HUGE drives, 1hr in and its at 16%
- I hope the RAID 1 system is easy to get back in order
- time: 4:12pm, 67% done with reformat
- Still have to install windows :(
- computer done installing at 5pm
- nothing comes up when i log in as the admin
- The problem i think is that the install disc that koch made does not have all the drivers that this computer needs
- I think this b/c although it is hooked up to the internet, i get no connection or response from the ethernet cable.
- Will try the drivers cd 2morrow or dl a fresh batch and send them over via usb stick
Acetic Acid
- while waiting on the computer, i will help brigette make MT's
- she is mixing up the acetic acid
- Note ok MT's: I will be using the BRB80T that Igor and I made earlier this week (monday?)
- it has been in the fridge all this time, so i think its still good
- mixed it up using the vortexer (koch mentioned that taxol can come out of soln if it sets) and am leaving it on the counter for ~20mins to warm up before adding it to the MT's
- Eventually i will remember the recipe for making MT's but for now I had to look it up
- As of this monday, its 2ul of tubulin and 198ul of BRB80T (premixed)
- Tubulin + brb80t mixed up
- Andy is messing with the microscope right now...he has the translator stage off, so I wont bother trying to look at the MT's until he gets back and gives me the ok
- looked at the MT's, no antifade. They are there. They fade in ~.25s
- Now to add acetic acid to the MT's
- Brigette made .273M Acetic Acid soln (w/DI H20)
- She wants to add 3ul of this soln to the ~200ul of tublules (technically only 195 since i took 5ul to look under the scope)
- Scratch that. We made a soln of 100MT to 3ul of acetic and 100ul MT to 1.5ul acetic
- Right now, brigette is looking at the 3ul aa soln
- They are still photobleaching quite fast, so it is really hard to tell if the tubes are breaking apart or not
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