User:Linh N Le/Notebook/2009/06/17/How to use the Autoclave: Difference between revisions

Revision as of 16:17, 17 June 2009

On the top of the autoclave is a round opening for DI water
The cap has a dipstick in it where you can check water levels
- Note: do not over fill the autoclave. There is a metal spigot inside the filling area that the water level must not rise above

Tubes should be placed in the round plastic screw cap containers
- The cap should be placed upon the top, but not screwed in
Pipette tip boxes should be opened and their lids on, but not airtight
All samples must have the special masking tape on them
- This tape is the manilla with white stripes on it
- Make a small tab at one end so that the tape can be easily removed for reuse of the container.
Place the samples onto the tray of the autoclave, leaving space between items

After the autoclave has been filled with water and loaded, turn on the green switch to "START"
Turn the bottom-most dial clockwise to the "FILL WATER" position
Watch inside the autoclave bottom as water starts to fill it
When the water reaches the door (There is a small notch before the door) set the bottom dial to "STE"
Close door and tighten (not too tight because you may damage the seal)
With the door closed, set the temperature gauge to 273F (121C)
Set the timer to 30mins (45mins if this is a cold start)
- Note: the pressure meter must be between 30-32 psi for the temperature to actually be 273F. If it is not, adjust the temperature gauge accordingly

After the timer dings set the bottom most knob to "EXH + DRY"
When the pressure gauge reaches 0 psi, turn the bottom most knob to the "0" position
Open door and remove samples

@@ Line 11: / Line 11: @@
 **This tape is the manilla with white stripes on it
 **Make a small tab at one end so that the tape can be easily removed for reuse of the container.
-*Place the samples into the tray of the autoclave, evenly spaced apart
+*Place the samples onto the tray of the autoclave, leaving space between items
 ==Setup==