User:Linh N Le/Notebook/2009/06/22: Difference between revisions
From OpenWetWare
Line 45: | Line 45: | ||
**Means I need .039971g in a ul | **Means I need .039971g in a ul | ||
***Meaning I need 1.598g in a 40ul soln | ***Meaning I need 1.598g in a 40ul soln | ||
*Thats wrong, new train of thought | |||
*Lets try to make .4L of 1M soln | |||
**I need 15.99884 g for .4L of water (4 deca liters) | |||
**divide by 10, 1.599884g for a 40ml | |||
===Making the Buffer== | |||
*We measured out the following | |||
**1mM EGTA (0.076g) | |||
**80mM PIPES (4.83g) | |||
**1mM MgCl<sub>2</sub> (.2mL) | |||
*For a 200mL stock of the Buffer (which Andy hates naming, but I will name KLB) | |||
*We titrated it with NaOH to raise the Ph to 6.89 | |||
**Used about 26ml of NaOH to get there | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} |
Revision as of 16:04, 22 June 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
To Do
Tubulin
BRB80
Ph Titrations
=Making the Buffer
|