User:Linh N Le/Notebook/2009/06/22: Difference between revisions
From OpenWetWare
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(→BRB80) |
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*Andy is looking over our new fancy [[Koch Lab:Inventory/pH meter|pH meter]] | *Andy is looking over our new fancy [[Koch Lab:Inventory/pH meter|pH meter]] | ||
**He is starting the calibration process | **He is starting the calibration process | ||
*BRB stands for [http://en.wikipedia.org/wiki/Britton-Robinson_buffer Britton-Robinson Buffer]{{SJK Comment|l=01:52, 23 June 2009 (EDT)|c=I can't tell if you're kidding! Check out this friendfeed thread: [http://friendfeed.com/the-life-scientists/59d2ea57/does-anyone-here-know-what-initials-brb80-stand what is BRB80?] | *BRB stands for [http://en.wikipedia.org/wiki/Britton-Robinson_buffer Britton-Robinson Buffer]{{SJK Comment|l=01:52, 23 June 2009 (EDT)|c=I can't tell if you're kidding! Check out this friendfeed thread: [http://friendfeed.com/the-life-scientists/59d2ea57/does-anyone-here-know-what-initials-brb80-stand what is BRB80?]}} | ||
**It is a "universal" buffer whose ph changes linearly with the amount of alkali that is added to it | **It is a "universal" buffer whose ph changes linearly with the amount of alkali that is added to it | ||
**It consists of: | **It consists of: | ||
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* [http://www.cytoskeleton.com/products/buffers/bst01.html Cytoskeleton PEM recipe] | * [http://www.cytoskeleton.com/products/buffers/bst01.html Cytoskeleton PEM recipe] | ||
* [http://openwetware.org/wiki/Koch_Lab:Protocols/Kinesin/BRB80_Acid_Pipes The actual recipe I used at Sandia] | * [http://openwetware.org/wiki/Koch_Lab:Protocols/Kinesin/BRB80_Acid_Pipes The actual recipe I used at Sandia] | ||
* [http://www.proweb.org/kinesin/Methods/motility.html Recipe on kinesin home page] (almost the same as cytoskeleton, but slightly lower pH] | * [http://www.proweb.org/kinesin/Methods/motility.html Recipe on kinesin home page] (almost the same as cytoskeleton, but slightly lower pH]}} | ||
===Ph Titrations=== | ===Ph Titrations=== | ||
*Recalling what little i remember from freshman chemistry, Andy has instructed me to create 40uL of a "normal" base of NaoH | *Recalling what little i remember from freshman chemistry, Andy has instructed me to create 40uL of a "normal" base of NaoH |
Revision as of 22:58, 22 June 2009
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To Do
Tubulin
BRB80
Ph Titrations
Making the Buffer
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