User:Linh N Le/Notebook/2009/06/22: Difference between revisions

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* Andy is back! He wants to try the kinesin again, maybe his expertise will allow us to succeed
* Andy is back! He wants to try the kinesin again, maybe his expertise will allow us to succeed
* Make BRB80
* Make BRB80
==Tubulin==
*Stumbled upon a [http://ylwang.umassmed.edu/protocol/pp/tubulin.htm site] that details how they get tubulin from cow's brains


==BRB80==
==BRB80==
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***.04M H<sub>3</sub>PO<sub>4</sub> (Phosphoric Acid)
***.04M H<sub>3</sub>PO<sub>4</sub> (Phosphoric Acid)
***.04M CH<sub>3</sub>CO0H (Acetic Acid)
***.04M CH<sub>3</sub>CO0H (Acetic Acid)
 
*The stuff we use is also known as PEM
**0.1 M PIPES (pH 6.95)
**2 mM EGTA
**1 mM MgSO4
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Revision as of 11:58, 22 June 2009

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To Do

  • Andy is back! He wants to try the kinesin again, maybe his expertise will allow us to succeed
  • Make BRB80

Tubulin

  • Stumbled upon a site that details how they get tubulin from cow's brains

BRB80

  • Since Andy and Koch are the only people in the lab that know how to make a buffer (I actually dont know if Ant can), we waited til Andy came back to try to make BRB80
    • Not to mention the fact that we are also running out of the stuff we got from Cytoskeleton (new shipment will be in this week)
  • Andy is looking over our new fancy Ph meter
  • BRB stands for Britton-Robinson Buffer
    • It is a "universal" buffer whose ph changes linearly with the amount of alkali that is added to it
    • It consists of:
      • .04M H3BO3 (Boric Acid)
      • .04M H3PO4 (Phosphoric Acid)
      • .04M CH3CO0H (Acetic Acid)
  • The stuff we use is also known as PEM
    • 0.1 M PIPES (pH 6.95)
    • 2 mM EGTA
    • 1 mM MgSO4
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