User:Linh N Le/Notebook/2009/06/23: Difference between revisions

From OpenWetWare
< User:Linh N Le‎ | Notebook‎ | 2009‎ | 06
Jump to navigationJump to search
Line 25: Line 25:
***2 mM EGTA
***2 mM EGTA
***1 mM Mg2SO4
***1 mM Mg2SO4
*Goldman Lab (Northwestern University)
**100 mM Pipes,pH 6.9
**1 mM MgCl2
**1 mM EGTA




Revision as of 13:29, 23 June 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To Do

  • Koch/Atlas group meeting at 3
  • Try out new buffer we made yesterday?
  • Try Kinesin?
  • Clean up lab

BRB80

  • Since there are so many ways to make this buffer, Koch wanted me to try to find different famous people's recipes for the buffer
    • People to look for, Block and Vale

Recipes

  • Hancock (Used by us as well)
    • 80 mM PIPES pH 6.85 (we use ph6.9)
    • 1 mM MgCl2
    • 1 mM EGTA
  • T.Salmon Labs
    • PM Buffer
      • 100 mM PIPES, pH 6.9
      • 2 mM EGTA
      • 1 mM Mg2SO4
  • Goldman Lab (Northwestern University)
    • 100 mM Pipes,pH 6.9
    • 1 mM MgCl2
    • 1 mM EGTA


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Linh_N_Le/Notebook/2009/06/23&oldid=318296"