User:Linh N Le/Notebook/2009/06/23: Difference between revisions

From OpenWetWare
< User:Linh N Le‎ | Notebook‎ | 2009‎ | 06
Jump to navigationJump to search
Line 93: Line 93:
***2 mM DTT
***2 mM DTT
***1 mM EGTA
***1 mM EGTA
***7 mMTaxol,
***7 mMTaxol
*[http://www.stanford.edu/group/blocklab/Valentine%20Fordyce%20et%20al%20NCB%202006.pdf Valentine, Fordyce, Block]
**polymerization buffer for brain tubulin
***62 mM PIPES at pH 6.9
***0.8 mM EGTA
***3.7 mM MgCl2
***0.9 mM GTP
***10% v/v DMSO




Revision as of 16:12, 23 June 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To Do

  • Koch/Atlas group meeting at 3
  • Try out new buffer we made yesterday?
  • Try Kinesin?
  • Clean up lab

BRB80

  • Since there are so many ways to make this buffer, Koch wanted me to try to find different famous people's recipes for the buffer
    • People to look for, Block and Vale
  • What we use for Cytoskeleton is called PEM and while PEM and BRB80 can be the same, they do not have to be
    • I did searches on both PEM and BRB80 and the following recipes are also reported by what they are called

Recipes


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Linh_N_Le/Notebook/2009/06/23&oldid=318392"