User:Linh N Le/Notebook/2009/06/23: Difference between revisions

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***pH 6.9
***pH 6.9
*[http://www.stanford.edu/group/blocklab/Block%20et%20al%20PNAS%202003.pdf Mathew Lang, Steve Block first author]
*[http://www.stanford.edu/group/blocklab/Block%20et%20al%20PNAS%202003.pdf Mathew Lang, Steve Block first author]
*Also used in [http://www.stanford.edu/group/blocklab/Rosenfeld%202003.pdf Rosenfield, Block Paper]
*Also used in [http://www.stanford.edu/group/blocklab/Guydosh%20and%20Block.pdf Guydosh Block Paper] with 10μM of Taxol
**Un-named buffer
**Un-named buffer
***80 mM Pipes (pH6.9)
***80 mM Pipes (pH6.9)
***50 mM potassium acetate
***50 mM potassium acetate
***4 mM MgCl2
***4 mM MgCl2
***2 mMDTT
***2 mM DTT
***1 mM EGTA
***1 mM EGTA
***7 mMTaxol,
***7 mMTaxol,


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Revision as of 16:08, 23 June 2009

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To Do

  • Koch/Atlas group meeting at 3
  • Try out new buffer we made yesterday?
  • Try Kinesin?
  • Clean up lab

BRB80

  • Since there are so many ways to make this buffer, Koch wanted me to try to find different famous people's recipes for the buffer
    • People to look for, Block and Vale
  • What we use for Cytoskeleton is called PEM and while PEM and BRB80 can be the same, they do not have to be
    • I did searches on both PEM and BRB80 and the following recipes are also reported by what they are called

Recipes