To Do
- Koch/Atlas group meeting at 3
- Try out new buffer we made yesterday?
- Try Kinesin?
- Clean up lab
BRB80
- Since there are so many ways to make this buffer, Koch wanted me to try to find different famous people's recipes for the buffer
- People to look for, Block and Vale
Recipes
- Hancock (Used by us as well)
- 80 mM PIPES pH 6.85 (we use ph6.9)
- 1 mM MgCl2
- 1 mM EGTA
- T.Salmon Labs
- PM Buffer
- 100 mM PIPES, pH 6.9
- 2 mM EGTA
- 1 mM Mg2SO4
- Goldman Lab (Northwestern University)
- 100 mM Pipes,pH 6.9
- 1 mM MgCl2
- 1 mM EGTA
- Cold Spring Harbor Labs
- PEM Buffer
- 0.1 M PIPES (pH 6.95)
- 2 mM EGTA
- 1 mM MgSO4
- Cytoskeleton
- PEM (General Tubulin Buffer)
- 80 mM Na-PIPES pH 6.9
- 1 mM MgCl2
- 1 mM EGTA
- Mitchison Lab (Harvard)
- Brinkley Buffer 1980 (BRB80)
- 80mM PIPES pH 6.8
- 1mM MgCl2
- 1mM EGTA
- "Cytoskeleton Buffer"
- 10mM MES pH 6.1
- 138mM KCl
- 3mM MgCl
- 2mM EGTA
- Notes: BRB80 is good for microtubules and 'cytoskeleton buffer' is good for both actin filaments and microtubules
- Sigma Aldrich
- PEM Buffer:
- 0.1M PIPES (Product No. P8203)
- 5 mM EGTA (Product No. E4378)
- 2mM MgCl2 · 6H2O (Product No. M0250)
- Bring to pH 6.8, using NaOH solution
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