User:Linh N Le/Notebook/2009/07/07: Difference between revisions
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==Camera== | ==Camera== | ||
{{SJK Comment|l=23:04, 7 July 2009 (EDT)|c=That's good to know that it works really well wired. One really important function is the remote camera control: Taking a picture while controlling and viewing from the computer. Does this work well over the wired?<br>[[User:Linh N Le|Linh N Le]] 01:08, 8 July 2009 (EDT)There is a button on the software that is supposed to control the camera remotely, but while it is wired, the button is un-clickable.}} | |||
*Koch wants to use the camera as a gel camera. | *Koch wants to use the camera as a gel camera. | ||
*He wonders how well it works wired | *He wonders how well it works wired | ||
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*It seems that I have the "essential" software installed | *It seems that I have the "essential" software installed | ||
*Looks like it does not come with a memory card, hope thats ok | *Looks like it does not come with a memory card, hope thats ok | ||
**The new battery is uncharged, using one from old cam | |||
*Setting up wireless | |||
**SSID Kochlab | |||
**security WPA-PSK-TKIP | |||
**key [[User:Andy Maloney|Andy Maloney]] 00:17, 8 July 2009 (EDT): <font color = red>Really? You put the key on the public wiki?</font> I know we are an open lab but don't you think that's a bit much? | |||
***[[User:Linh N Le|Linh N Le]] 01:07, 8 July 2009 (EDT)Oh shoot! I'm sorry, i totally spaced that I did that. I just wanted to document what I wrote down so that if the camera didn't connect it could have been traced to a bad password. I gotta keep reminding myself that this is a ''public'' wiki | |||
*Got that setup but when trying to connect this battery also died | |||
**May need to wait for a charge tomorrow | |||
==Kinesin Activity Assay== | ==Kinesin Activity Assay== | ||
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*Right now, we are going to polymerize some non-labeled tubes | *Right now, we are going to polymerize some non-labeled tubes | ||
*Brigette is writing up some procedures which I hope to copy from her and paste in my notebook | *Brigette is writing up some procedures which I hope to copy from her and paste in my notebook | ||
==Procedure== | ===Procedure=== | ||
*Taking note of what we did as we do it: | *Taking note of what we did as we do it: | ||
*1 aliquot of the unlabeled tubes | *1 aliquot of the unlabeled tubes | ||
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*We let the samples sit for ~10mins to allow the reactions to complete (since adding the dye and quencher stop the reactions all together) | *We let the samples sit for ~10mins to allow the reactions to complete (since adding the dye and quencher stop the reactions all together) | ||
*Turns out it is over diluted and Brigette didn't get any useful readings from it | *Turns out it is over diluted and Brigette didn't get any useful readings from it | ||
===Trial 2=== | ====Trial 2==== | ||
*We are redoing it, but w/o any naked brb80 | *We are redoing it, but w/o any naked brb80 | ||
*Samples: | *Samples: |
Revision as of 22:08, 7 July 2009
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To Do
CameraSJK 23:04, 7 July 2009 (EDT)
Round 2
Kinesin Activity Assay
Procedure
Trial 2
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