User:Linh N Le/Notebook/2009/07/16: Difference between revisions

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**This is 1x PBS
**This is 1x PBS
*This needs to be stored @ room temp, so I will put it with the Popping buffer near Brigette's work area
*This needs to be stored @ room temp, so I will put it with the Popping buffer near Brigette's work area
===update===
*So the PBS exploded in the autoclave, leaving alot of salt everywhere
*I would say about 20ml of volume was lost
**Koch worries that if it was water that evaporated instead of actual liquid splashing, then we could have a problem with concentrations
***There is salt residue everywhere so i am lead to believe that its mostly the liquid and that the concentration is ok
*I will need to clean the autoclave and we do have special autoclave cleaning powder stuff
**Tomorrow I will give it a really good cleaning, removing water and all that jazz
***We may want to look into buying more cleaning powder stuff too
==Heat Plate==
==Heat Plate==
*Andy asked me to take a look at the Hot Plate from Fischer that died quite a while ago
*Andy asked me to take a look at the Hot Plate from Fischer that died quite a while ago

Revision as of 16:10, 16 July 2009

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To Do

  • More OT/DNA stuff?
  • Fuss with the stupid table again?

PBS Buffer

  • I made 1L of PBS buffer
  • 0.01 M phosphate buffer
  • 0.0027 M potassium chloride
  • 0.137 M sodium chloride
  • pH 7.47
    • This is 1x PBS
  • This needs to be stored @ room temp, so I will put it with the Popping buffer near Brigette's work area

update

  • So the PBS exploded in the autoclave, leaving alot of salt everywhere
  • I would say about 20ml of volume was lost
    • Koch worries that if it was water that evaporated instead of actual liquid splashing, then we could have a problem with concentrations
      • There is salt residue everywhere so i am lead to believe that its mostly the liquid and that the concentration is ok
  • I will need to clean the autoclave and we do have special autoclave cleaning powder stuff
    • Tomorrow I will give it a really good cleaning, removing water and all that jazz
      • We may want to look into buying more cleaning powder stuff too

Heat Plate

  • Andy asked me to take a look at the Hot Plate from Fischer that died quite a while ago
  • He took it apart and could not figure it out (which worries me since he's the mechanical genius in these here parts)
  • Right now, what I see is that there are 2 variable resistors that go into a transformer that is powered by the AC outlet.
  • One of the resistors goes to the heating element and the other goes into a motor that spins a pair of square magnets
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