User:Linh N Le/Notebook/2009/08/28: Difference between revisions
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* Andy, Brigette and I made 205ml of PEM a month or 2 ago and its getting low | * Andy, Brigette and I made 205ml of PEM a month or 2 ago and its getting low | ||
* I will try to make 200ml more and follow the exact steps I took [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/06/22 last time] | * I will try to make 200ml more and follow the exact steps I took [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/06/22 last time] | ||
* I will need NaOH and possibly HCL to titrate the buffer | |||
** We should have plenty of normal soln left from earlier buffer makings | |||
** We have 4 tubes of NaOH but no HCL, lets hope I dont screw up the titration | |||
===Procedure=== | |||
*Following what I did last time, I want the following: | |||
** 1mM MgCl2 | |||
** 1mM EGTA | |||
** 80mM PIPES | |||
*I am using the following chemicals: | |||
**1M MgCl2 in 50mL falcon tube made 2 years ago ( we used it in the motility assay yesterday and it was fine) | |||
***For 1mM in 200ml, I want .2ml (200uL) | |||
**EGTA powder, working weight 380.35 | |||
**For 1mM in 200ml i need .07607g | |||
**PIPES powder, working weight 302.37 | |||
**For 80mM in 200ml, I need 4.83792g | |||
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Revision as of 09:54, 28 August 2009
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