User:Linh N Le/Notebook/2009/08/28

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(PEM)
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* Andy, Brigette and I made 205ml of PEM a month or 2 ago and its getting low
* Andy, Brigette and I made 205ml of PEM a month or 2 ago and its getting low
* I will try to make 200ml more and follow the exact steps I took [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/06/22 last time]
* I will try to make 200ml more and follow the exact steps I took [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/06/22 last time]
 +
* I will need NaOH and possibly HCL to titrate the buffer
 +
** We should have plenty of normal soln left from earlier buffer makings
 +
** We have 4 tubes of NaOH but no HCL, lets hope I dont screw up the titration
 +
===Procedure===
 +
*Following what I did last time, I want the following:
 +
** 1mM MgCl2
 +
** 1mM EGTA
 +
** 80mM PIPES
 +
*I am using the following chemicals:
 +
**1M MgCl2 in 50mL falcon tube made 2 years ago ( we used it in the motility assay yesterday and it was fine)
 +
***For 1mM in 200ml, I want .2ml (200uL)
 +
**EGTA powder, working weight 380.35
 +
**For 1mM in 200ml i need .07607g
 +
**PIPES powder, working weight 302.37
 +
**For 80mM in 200ml, I need 4.83792g
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Revision as of 11:54, 28 August 2009

Project name Main project page
Previous entry      

To Do

  • Write my last official notebook entry as a KochLab member :(
  • We are running out of PEM, so I would like to make some more before I go
  • We got motility working yesterday, so Andy may want to continue to pursue that

Inventory

  • Brian had to leave, but i did give him a crash course on how to use the inventory page
  • Most of the pages are self-explanatory
  • Also note that chemicals and software inventory require an update to the google docs page
  • The optics section of the inventory is blank
    • My idea was to have it as a static page that just listed important opto-mechanics
    • Perhaps we can have a check in-check out system so we can keep track of who took what where and when

PEM

  • Andy, Brigette and I made 205ml of PEM a month or 2 ago and its getting low
  • I will try to make 200ml more and follow the exact steps I took last time
  • I will need NaOH and possibly HCL to titrate the buffer
    • We should have plenty of normal soln left from earlier buffer makings
    • We have 4 tubes of NaOH but no HCL, lets hope I dont screw up the titration

Procedure

  • Following what I did last time, I want the following:
    • 1mM MgCl2
    • 1mM EGTA
    • 80mM PIPES
  • I am using the following chemicals:
    • 1M MgCl2 in 50mL falcon tube made 2 years ago ( we used it in the motility assay yesterday and it was fine)
      • For 1mM in 200ml, I want .2ml (200uL)
    • EGTA powder, working weight 380.35
    • For 1mM in 200ml i need .07607g
    • PIPES powder, working weight 302.37
    • For 80mM in 200ml, I need 4.83792g
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