User:Lu Wang/Notebook/Team Allergy/2010/06/17: Difference between revisions
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== | ==Gel Electrophoresis== | ||
Today, we loaded gel slots to run our 6 DNA samples against a 1kb ladder. The order of our slots were: ladder, ladder, sense 1, sense 1, sense 2, sense 2, sense 3, sense 3, antisense 1, antisense 1, antisense 2, antisense 2, antisense3, and antisense 3. We had to load two slots because there was more than 50 microliters of DNA and it would not fit into one slot. We also loaded two ladders. We ran the gel for 30 minutes at 100 volts. | |||
Revision as of 07:22, 17 June 2010
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Gel ElectrophoresisToday, we loaded gel slots to run our 6 DNA samples against a 1kb ladder. The order of our slots were: ladder, ladder, sense 1, sense 1, sense 2, sense 2, sense 3, sense 3, antisense 1, antisense 1, antisense 2, antisense 2, antisense3, and antisense 3. We had to load two slots because there was more than 50 microliters of DNA and it would not fit into one slot. We also loaded two ladders. We ran the gel for 30 minutes at 100 volts.
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