User:Lu Wang/Notebook/Team Allergy/2010/06/21: Difference between revisions
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==Faculty Presentation 2== | ==Faculty Presentation 2== | ||
Team Vector, Team Flavor, Team Allergy, and Team Fence presented their progress from the previous week of June 14th to June 18th. A copy of the presentation can be found [https://docs.google.com/present/edit?id=0AXjFW9ZXw2djZGN3MzZrdzVfMTdjMzJ2aHpnaw&hl=en | Team Vector, Team Flavor, Team Allergy, and Team Fence presented their progress from the previous week of June 14th to June 18th. A copy of the presentation can be found [https://docs.google.com/present/edit?id=0AXjFW9ZXw2djZGN3MzZrdzVfMTdjMzJ2aHpnaw&hl=en here]. | ||
Some questions and comments that came from the faculty for the various teams. Obviously, these are incomplete, but I will request input from members of the other team. | |||
Team Vector | |||
- how to insert oligos into their vectors? | |||
- do the biobricks code for any unusual proteins or cause any change/damage to the plants? | |||
Team Flavor | |||
Team Allergy | |||
- what happens when we remove the allergen proteins? | |||
- what were the results from other similar experiements? | |||
Team Fence | |||
Our next faculty presentation will be in one week on June 28th. | |||
==Strawberry hpRNA Interference== | |||
The gene coding for Fra a 1 has a 150bp exon (we're going to call it exon fra1), followed by a 150bp intron (intron fra1) and 300 exon (exon fra2). The next time we isolate mRNA from strawberries, we are going to isolate mRNA for exon fra2 only. Including an intron in the sense/antisense of the hpRNA may cause the plant to have multiple targets for interference, and we only want to shut down genes coding for fra a 1. Therefore, we will only use one exon. We will use exon fra 2 because we need a hpRNA at least 1000bps long, and exon fra1 may be too short. | |||
Last week, we attempted to isolate mRNA include... | |||
==PCR with Wildtype Arabadopsis== | |||
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Revision as of 14:06, 21 June 2010
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Faculty Presentation 2Team Vector, Team Flavor, Team Allergy, and Team Fence presented their progress from the previous week of June 14th to June 18th. A copy of the presentation can be found here. Some questions and comments that came from the faculty for the various teams. Obviously, these are incomplete, but I will request input from members of the other team. Team Vector - how to insert oligos into their vectors? - do the biobricks code for any unusual proteins or cause any change/damage to the plants? Team Flavor Team Allergy - what happens when we remove the allergen proteins? - what were the results from other similar experiements? Team Fence Our next faculty presentation will be in one week on June 28th. Strawberry hpRNA InterferenceThe gene coding for Fra a 1 has a 150bp exon (we're going to call it exon fra1), followed by a 150bp intron (intron fra1) and 300 exon (exon fra2). The next time we isolate mRNA from strawberries, we are going to isolate mRNA for exon fra2 only. Including an intron in the sense/antisense of the hpRNA may cause the plant to have multiple targets for interference, and we only want to shut down genes coding for fra a 1. Therefore, we will only use one exon. We will use exon fra 2 because we need a hpRNA at least 1000bps long, and exon fra1 may be too short. Last week, we attempted to isolate mRNA include...
PCR with Wildtype Arabadopsis | |
