User:Lu Wang/Notebook/Team Allergy/2010/07/26
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ProceduresLast week, we finished ligation together Sense and Intron parts of the hpRNA and transformed them into E. coli. This week, we will extract the plasmids, add in the antisense part, and transform. Procedures today...
We also need to check which parts of the sequence on the team page corresponds to the sense/antisense parts of the gene. There may have been mistakes in the past. ResultsCulture We cultured 38 colonies. All grew but one LTP+PAL and Ger+Pal grew. We labeled the cultures 1- 38 for simplicity sake. 1-6: LTP + PDk 7-16: Miniprep Here are the concentrations of the miniprepped plasmid.
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