User:Mackenzie K. Kelley/Notebook/Experimental Biochemistry/2017/09/13

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Objective

  • Dialysis on a AuNP solution to determine:
  • how big the AuNP complexes are
  • how strongly the stabilizer is bound to the AuNP
  • if we can change the stabilizer
  • how quickly the molecules migrate through the tubing

Description

  • Stabilizer exchange
  • prepared and ran dialysis on two dialysis tubes: 1 with DI water and 25 mM HdMe2ImCl, 1 with AuNP solution and 25 mM HdMe2ImCl (Cysteine in BSA 90 ratio Au:AA)
  • Ever 30 minutes, remove 1 mL of solution from jar and measure the absorbance at 211 nm using the spectrophotometer

note: we were taking the solution out of the dialysis bag itself

  • Other exchanges
  • Using same procedure to prepare dialysis tubes, prepared a total of 4 dialysis tubes.
  • Dialysis 3 mL AuNP stabilized with basic AA against Glycine buffer
  • Dialysis 3 mL AuNP stabilized with basic AA against water
  • Dialysis 3 mL AuNP stabilized with BSA against Glycine buffer
  • Dialysis 3 mL AuNP stabilized with BSA against water


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