User:Mackenzie K. Kelley/Notebook/Experimental Biochemistry/2017/09/20: Difference between revisions
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== | ==Objective== | ||
* | *Run SDS page on Amino acid solutions | ||
==Description== | |||
*Prepared solutions to be loaded in wells (BSA stock with Glycine-HCl buffer, tryptophan stock solution, AuNP with BSA, and AuNP with tryptophan, polylysine) | |||
*10 ul of all listen solutions were added to 10 ul of SDS buffer | |||
*samples were heated at 95C for 5 minutes and then 20ul of each were added to wells of electrophoresis gel | |||
*Electrophoresis cell was prepared by filling chamber with buffer | |||
*Electrophoresis was run for 30 mins at 200V | |||
*Gel was then stained in fixative solution, stain solution, and then destain solution | |||
Revision as of 16:18, 21 September 2017
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