User:Mackenzie L. Cowell/IGEM 2007 part fab protocol
Overview
The 2007 DNA part kit will be comprised of around 1500 parts contained on 4 384-well plates. We need to fabricate 150 copies of the kit, producing a total of 600 384-well plates. The parts are contained on plasmids in cultures of E. coli frozen in glycerol stocks in 96-deep-well (96d) plates in -80 freezers at the registry. We are going to inoculate 96d plates from the freezer stocks and give them to Ed's lab for incubation and then miniprepping in 4 groups of 4, for a total of 16 96d plates. For every 4 that we get back we will use the EPM to compose the miniprepped DNA into 9 384d intermediate source plates (SP), of which we will give 3 back to Ed's lab to use with the PMP to transfer 3.5 uL of DNA into 150 384-well kit plates. This will happen 4 times, producing an uncollated set of the 600 384-well plates for the 150 07 DNA part kits.
Supply List
The 07 kits will be comprised of no more than 4 384-well plates (1536 parts), so to make 150 kits, we'll need:
- 32 96-deep-well square-bottom plates
- 16 for Ed's miniprep, will be sources for the intermediate SPs
- 16 for miniprepping for Robotic Assembly supplies
- 16 96-deep-well round-bottom plates (for 2 glycerol stocks of each new registry freezer plate)
- 56 384-deep-well plates
- 16 for Antibiotic Testing
- 4 for Robotic Assembly supplies
- 36 for intermediate Source Plates for kit fab with the PMP
- 600 384-well plates, for kits (4 per kit)
- 672 foil covers
656 plastic covers(AB test plates don't need plastic covers)- 15 rolls label tape
- 32 poxes of tips
(Items that are crossed out have been delivered to the Registry and are stored in 314)