User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions
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Rest of procedure followed as written. | Rest of procedure followed as written. | ||
====Citrate-AuNP Procedure==== | ====Citrate-AuNP Procedure==== | ||
[HAuCl<sub>4</sub>]=0.249Mm<BR.> | [HAuCl<sub>4</sub>]=0.249Mm<BR.> |
Revision as of 10:12, 16 October 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
August 28, 2013ObjectiveExecute procedures from from Dr Harting's AU Biomaterials Design Lab Notebook ProceduresBSA-AuNP Procedure[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> Final [HAuCl4] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> <br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> <br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> <br.> Rest of procedure followed as written. Citrate-AuNP Procedure[HAuCl4]=0.249Mm<BR.> Sodium citrate=1.01%<br.> <br.> Rest of procedure followed as written.<br.> <br.> Final volume AuNPs: 25.6mL<br.> <br.> Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> Data<br.> Absorbance spectra for AuNPs synthesized by citrate reduction.<br.>
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