User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry: Fall 2013</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==August 28, 2013==
* Insert content here...
===Objective===
Execute procedures from from [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 Dr Harting's AU Biomaterials Design Lab Notebook]
 
===Procedures===
====BSA-AuNP Procedure====
[HAuCl<sub>4</sub>]=2.54nM<br.>
[BSA]=15.6μM<br.>
Final [HAuCl<sub>4</sub>]: 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl<sub>4</sub>]<br.>
<br.>
(2.54mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.404mM HAuCl<sub>4</sub> final)(V)<br.>
V=1.809mL BSA<br.>
<br.>
Combine 1mL HAuCl<sub>4</sub> with 1.810mL BSA.<br.>
<br.>
Rest of procedure followed as written.
 
====Citrate-AuNP Procedure====
[HAuCl<sub>4</sub>]=0.249Mm<BR.>
Sodium citrate=1.01%<br.>
<br.>
Rest of procedure followed as written.<br.>
<br.>
Final volume AuNPs: 25.6mL<br.>
<br.>
Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.>
 
===Data===
[[Image:2013_0828_citrate_AuNPs.png]]<br.>
Absorbance spectra for AuNPs synthesized by citrate reduction.<br.>
*Relevant wavelengths and absorption:
**λ<sub>spr</sub>=518.5nm<br.>
**A<sub>spr</sub>=0.143<br.>
**A<sub>450</sub>=0.091<br.>
**A<sub>spr</sub>/A<sub>450</sub>=0.143/0.091=1.57<br.>
*According to the Supporting Information from [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra']:<br.>
**AuNP diameter=12nm<br.>
**ε<sub>450</sub> for 12nm AuNPs=1.09E+08<br.>
*Using Beer's Law to calculate concentration:<br.>
**C=8.349E-10M or 0.8349nM<br.>
*Accounting for the 10x dilution factor used in taking absorbance measurements:<br.>
**C=8.349nM<br.>
 
 
 
 
 
 
 




Revision as of 10:26, 16 October 2013

Experimental Biological Chemistry: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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August 28, 2013

Objective

Execute procedures from from Dr Harting's AU Biomaterials Design Lab Notebook

Procedures

BSA-AuNP Procedure

[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> Final [HAuCl4]: 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> <br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> <br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> <br.> Rest of procedure followed as written.

Citrate-AuNP Procedure

[HAuCl4]=0.249Mm<BR.> Sodium citrate=1.01%<br.> <br.> Rest of procedure followed as written.<br.> <br.> Final volume AuNPs: 25.6mL<br.> <br.> Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.>

Data

<br.> Absorbance spectra for AuNPs synthesized by citrate reduction.<br.>

  • Relevant wavelengths and absorption:
    • λspr=518.5nm<br.>
    • Aspr=0.143<br.>
    • A450=0.091<br.>
    • Aspr/A450=0.143/0.091=1.57<br.>
  • According to the Supporting Information from 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra':<br.>
    • AuNP diameter=12nm<br.>
    • ε450 for 12nm AuNPs=1.09E+08<br.>
  • Using Beer's Law to calculate concentration:<br.>
    • C=8.349E-10M or 0.8349nM<br.>
  • Accounting for the 10x dilution factor used in taking absorbance measurements:<br.>
    • C=8.349nM<br.>






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