User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions
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Rest of procedure followed as written.<br.> | Rest of procedure followed as written.<br.> | ||
<br.> | <br.> | ||
Final volume AuNPs: | Final volume AuNPs: 25.6mL<br.> | ||
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Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> | Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> | ||
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**ε<sub>450</sub> for 12nm AuNPs=1.09E+08<br.> | **ε<sub>450</sub> for 12nm AuNPs=1.09E+08<br.> | ||
*Using Beer's Law to calculate concentration:<br.> | *Using Beer's Law to calculate concentration:<br.> | ||
**C= | **C=8.349E-10M or 0.8349nM<br.> | ||
*Accounting for the 10x dilution factor used in taking absorbance measurements:<br.> | *Accounting for the 10x dilution factor used in taking absorbance measurements:<br.> | ||
**C= | **C=8.349nM<br.> | ||
Revision as of 05:50, 2 September 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
August 28, 2013ObjectiveExecute procedures from from Dr Harting's AU Biomaterials Design Lab Notebook ProceduresBSA-AuNP Procedure[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> Final [HAuCl4] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> <br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> <br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> <br.> Rest of procedure followed as written. Citrate-AuNP Procedure[HAuCl4]=0.249Mm<BR.> Sodium citrate=1.01%<br.> <br.> Rest of procedure followed as written.<br.> <br.> Final volume AuNPs: 25.6mL<br.> <br.> Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> Data<br.> Absorbance spectra for AuNPs synthesized by citrate reduction.<br.>
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