User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03

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(Stock Solutions and Dilutions)
(Stock Solutions and Dilutions)
Line 24: Line 24:
Dilutions to make, each with total volumes of 1mL:<br.>
Dilutions to make, each with total volumes of 1mL:<br.>
|<u>Adenosine solution concentrations (M)
|<u>Adenosine solution concentrations (M)</u>
|<u>Volume stock solution added (L)
|<u>Volume stock solution added (L)</u>
|<u>Inosine solution concentrations (M)
|<u>Inosine solution concentrations (M)</u>
|<u>Volume of stock solution added (L)
|<u>Volume of stock solution added (L)<u/>

Revision as of 15:09, 3 September 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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September 3, 2013


Execute Dr Hartings' procedureby making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW:267.24g/mol
Inosine MW:268.2g/mol

Original stock solutions:
Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M
Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M

Dilutions to make, each with total volumes of 1mL:

Adenosine solution concentrations (M) Volume stock solution added (L) Inosine solution concentrations (M) Volume of stock solution added (L)<u/>
3.00x10-5 3.66x10-6 4.80x10-5 6.08x10-6
2.50x10-5 3.05x10-6 4.00x10-5 5.06x10-6
2.00x10-5 2.44x10-6 3.20x10-5 4.05x10-6
1.50x10-5 1.83x10-6 2.40x10-5 3.04x10-6
1.00x10-5 1.22x10-6 1.60x10-5 2.03x10-6
0.50x10-5 6.10x10-7 0.80x10-5 1.01x10-6
RANDOM: 0.25x10-5 3.05x10-7 RANDOM: 0.40x10-5 5.06x10-7


Absorption Spectra


Calculations and Analysis

Determine the standard deviation for your data points.
Determine the confidence interval for 90% and 95% confidence.
Determine if any data can be ruled out using a Q-test.

Calibration Curves

Determining Unknown's Concentration


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