User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/24: Difference between revisions
From OpenWetWare
No edit summary |
|||
Line 19: | Line 19: | ||
# After 30 minutes, remove two samples: | # After 30 minutes, remove two samples: | ||
#* SDS-PAGE Samples (for TOMORROW) | #* SDS-PAGE Samples (for TOMORROW) | ||
#*# 10uL of the reaction sample, diluted to 1mL with the Glycine-HCl buffer | #*# '''10uL''' of the reaction sample, diluted to 1mL with the Glycine-HCl buffer | ||
#*# 10uL of this diluted sample, mixed with 10uL of the SDS-PAGE running buffer | #*# 10uL of this diluted sample, mixed with 10uL of the SDS-PAGE running buffer | ||
#*# Store all of your SDS-PAGE samples in the fridge | #*# Store all of your SDS-PAGE samples in the fridge | ||
#* UV-Vis Samples (for TODAY) | #* UV-Vis Samples (for TODAY) | ||
#*# | #*# '''0.75mL''' of the reaction sample, add 0.75mL of 1M perchloric acid to precipitate the remaining hemoglobin | ||
#*# After 1 hour, centrifuge for 15 minutes to remove solid (uncleaved hemoglobin) from solution | #*# After 1 hour, centrifuge for 15 minutes to remove solid (uncleaved hemoglobin) from solution | ||
#*# Measure the absorption (specifically note 280nm) to determine the protein concentration in solution | #*# Measure the absorption (specifically note 280nm) to determine the protein concentration in solution | ||
Line 29: | Line 29: | ||
#*#*Use Hemoglobin as reference | #*#*Use Hemoglobin as reference | ||
# Repeat Step 3 every 30 minutes for 2 hours. | # Repeat Step 3 every 30 minutes for 2 hours. | ||
===Data=== | ===Data=== |
Revision as of 10:28, 24 September 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
September 24, 2013ObjectivePepsin cleavage of hemoglobin according to this procedure. Description
DataNotes[[Category:]]
|