User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/25: Difference between revisions
From OpenWetWare
Line 14: | Line 14: | ||
===Description=== | ===Description=== | ||
Gel Electrophoresis: Lane Assignments | <u>Gel Electrophoresis: Lane Assignments</u> | ||
#100x Diluted Hemoglobin & Dye | #100x Diluted Hemoglobin & Dye | ||
#Pepsin 1 | #Pepsin 1 | ||
Line 28: | Line 28: | ||
#100x Diluted Hemoglobin & Pepsin | #100x Diluted Hemoglobin & Pepsin | ||
JMC's 12. 100x Diluted Hemoglobin & Pepstatin | JMC's 12. 100x Diluted Hemoglobin & Pepstatin | ||
<u>UV-vis Sample Prep</u> | |||
*Combine 0.75mL 1M perchloric acid with 0.75mL hemoglobin&pepsin and 0.75mL hemoglobin*pepstatin samples that sat overnight | |||
*Allow reaction to proceed for one hour (until 2:16pm) | |||
*Centrifuge for 15 minutes | |||
*Take supernatant, run absorbance measurements | |||
===Data=== | ===Data=== |
Revision as of 10:18, 25 September 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
September 25, 2013ObjectiveRun SDS-PAGE to observe the amount of hemoglobin leftover from yesterday. DescriptionGel Electrophoresis: Lane Assignments
JMC's 12. 100x Diluted Hemoglobin & Pepstatin UV-vis Sample Prep
DataNotes[[Category:]]
|