User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/01

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==Date==
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==October 1, 2013==
===Objective===
===Objective===
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#UV-Vis Absorbance of reactants, catalysts, and products
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#*Make 10mL stock solution of luminol at appropriate dilution
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#Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer
===Description===
===Description===
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#
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<u>UV-vis Samples</u><br.>
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*Blank: TRIS buffer
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*Horseradish peroxidase
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*H<sub>2</sub>O<sub>2</sub>
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*20x luminol
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*10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H<sub>2</sub>O<sub>2</sub> catalyzed by 333uL HRP)
===Data===
===Data===
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*
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<u>Absorbance Measurements</u><br.>
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[[Image:2013_1001_absorbance.PNG]]<br.>
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<u>Beer's Law concentration calculations</u><br.>
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[[Image:2013_1001_beer's_law_calcs.PNG]]<br.>
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===Notes===
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<u>Kinetic Measurements</u>
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===Notes===
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[[Category:]]
 
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[[Category:Miscellaneous]]
 

Current revision

Experimental Biological Chemistry: Fall 2013 Main project page
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October 1, 2013

Objective

  1. UV-Vis Absorbance of reactants, catalysts, and products
    • Make 10mL stock solution of luminol at appropriate dilution
  2. Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer

Description

UV-vis Samples

  • Blank: TRIS buffer
  • Horseradish peroxidase
  • H2O2
  • 20x luminol
  • 10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H2O2 catalyzed by 333uL HRP)

Data

Absorbance Measurements
Image:2013_1001_absorbance.PNG
Beer's Law concentration calculations
Image:2013_1001_beer's_law_calcs.PNG

Kinetic Measurements


Notes


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