User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/01

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<u>Kinetic Measurements</u>

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Experimental Biological Chemistry: Fall 2013 Main project page
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October 1, 2013


  1. UV-Vis Absorbance of reactants, catalysts, and products
    • Make 10mL stock solution of luminol at appropriate dilution
  2. Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer


UV-vis Samples

  • Blank: TRIS buffer
  • Horseradish peroxidase
  • H2O2
  • 20x luminol
  • 10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H2O2 catalyzed by 333uL HRP)


Absorbance Measurements
Beer's Law concentration calculations

Kinetic Measurements


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