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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry: Fall 2013</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry: Fall 2013</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Date==
==October 1, 2013==


===Objective===
===Objective===
 
#UV-Vis Absorbance of reactants, catalysts, and products
#*Make 10mL stock solution of luminol at appropriate dilution
#Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer


===Description===
===Description===
#
<u>UV-vis Samples</u><br.>
*Blank: TRIS buffer
*Horseradish peroxidase
*H<sub>2</sub>O<sub>2</sub>
*20x luminol
*10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H<sub>2</sub>O<sub>2</sub> catalyzed by 333uL HRP)


===Data===
===Data===
*
<u>Absorbance Measurements</u><br.>
[[Image:2013_1001_absorbance.PNG]]<br.>
<u>Beer's Law concentration calculations</u><br.>
[[Image:2013_1001_beer's_law_calcs.PNG]]<br.>


===Notes===
<u>Kinetic Measurements</u>




===Notes===


[[Category:]]
[[Category:Miscellaneous]]




Latest revision as of 23:24, 26 September 2017

Experimental Biological Chemistry: Fall 2013 Main project page
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October 1, 2013

Objective

  1. UV-Vis Absorbance of reactants, catalysts, and products
    • Make 10mL stock solution of luminol at appropriate dilution
  2. Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer

Description

UV-vis Samples<br.>

  • Blank: TRIS buffer
  • Horseradish peroxidase
  • H2O2
  • 20x luminol
  • 10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H2O2 catalyzed by 333uL HRP)

Data

Absorbance Measurements<br.> <br.> Beer's Law concentration calculations<br.> <br.>

Kinetic Measurements


Notes


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