User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/01

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Experimental Biological Chemistry: Fall 2013 Main project page
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October 1, 2013

Objective

  1. UV-Vis Absorbance of reactants, catalysts, and products
    • Make 10mL stock solution of luminol at appropriate dilution
  2. Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer

Description

UV-vis Samples

  • Blank: TRIS buffer
  • Horseradish peroxidase
  • H2O2
  • 20x luminol
  • 10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H2O2 catalyzed by 333uL HRP)

Data

Absorbance Measurements
Image:2013_1001_absorbance.PNG
Beer's Law concentration calculations
Image:2013_1001_beer's_law_calcs.PNG



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