User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/02: Difference between revisions
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===Objective=== | ===Objective=== | ||
#Chemiluminescence of luminol oxidation reaction initiated by stopped flow mixer | |||
#[REDO] Kinetics of luminol oxidized by changes in absorption spectrum in stopped flow mixer | |||
===Description=== | |||
<u>Chemiluminescence</u> | |||
*Tube 1 (10mL): 0.5mM luminol, 5nM HRP | *Tube 1 (10mL): 0.5mM luminol, 5nM HRP | ||
*Tube 2 (10mL): 5mM H<sub>2</sub>O<sub>2</sub> (from stock 12.8mM) | *Tube 2 (10mL): 5mM H<sub>2</sub>O<sub>2</sub> (from stock 12.8mM) | ||
<u>Redo Kinetics</u> | |||
*Determine appropriate concentrations of reagents (HRP needs to be 10000x less concentrated than luminol, to slow reaction rate) | |||
*Double concentrations to increase absorbance, to monitor bigger changes | |||
===Data=== | ===Data=== | ||
<u>Kinetics: HAT Group's Data from 10/01/2013</u><br.> | |||
[[Image:2013_1001_HAT_kinetics_abs.PNG]]<br.> | |||
===Notes=== | ===Notes=== | ||
*Concentrations used yesterday were too low. Dr Hartings & HAT group used higher concentrations which yielded the oxidized luminol kinetics absorption spectra shown above. | |||
*HRP must be contaiminated: no groups seeing any signal from a variety of concentrations. Dr Hartings will make new HRP solution. | |||
Revision as of 12:06, 2 October 2013
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October 2, 2013Objective
DescriptionChemiluminescence
Redo Kinetics
DataKinetics: HAT Group's Data from 10/01/2013<br.> <br.> Notes
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