User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/02

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===Objective===
===Objective===
 +
<u>Chemiluminescence</u>
*Tube 1 (10mL): 0.5mM luminol, 5nM HRP
*Tube 1 (10mL): 0.5mM luminol, 5nM HRP
*Tube 2 (10mL): 5mM H<sub>2</sub>O<sub>2</sub> (from stock 12.8mM)
*Tube 2 (10mL): 5mM H<sub>2</sub>O<sub>2</sub> (from stock 12.8mM)
 +
 +
<u>Redo Kinetics</u>
 +
*Determine appropriate concentrations of reagents (HRP needs to be 10000x less concentrated than luminol, to slow reaction rate)
 +
*Double concentrations to increase absorbance, to monitor bigger changes
===Description===
===Description===

Revision as of 12:25, 2 October 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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October 2, 2013

Objective

Chemiluminescence

  • Tube 1 (10mL): 0.5mM luminol, 5nM HRP
  • Tube 2 (10mL): 5mM H2O2 (from stock 12.8mM)

Redo Kinetics

  • Determine appropriate concentrations of reagents (HRP needs to be 10000x less concentrated than luminol, to slow reaction rate)
  • Double concentrations to increase absorbance, to monitor bigger changes

Description

Data

Notes


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