User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/16: Difference between revisions
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#*Stock [BSA]:15.0uM | #*Stock [BSA]:15.0uM | ||
#*Final [HAuCl<sub>4</sub>]= 90×[BSA]=90×15.0uM=1350μM=1.350mM=final [HAuCl<sub>4</sub>] | #*Final [HAuCl<sub>4</sub>]= 90×[BSA]=90×15.0uM=1350μM=1.350mM=final [HAuCl<sub>4</sub>] | ||
#**(2.33mM HAuCl<sub>4</sub> initial)( | #**(2.33mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.350mM HAuCl<sub>4</sub> final)(V) | ||
#**V= | #**V=1.73mL BSA<br.> | ||
#*Combine 1mL | #*Combine 1mL HAuCl<sub>4</sub> and 1.73mL BSA, dilute with distilled water up to 10mL | ||
#Transfer solution to a test tube and cap with aluminum foil | #Transfer solution to a test tube and cap with aluminum foil | ||
#Heat in oven at 80C | #Heat in oven at 80C |
Revision as of 12:20, 16 October 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
October 16, 2013Objective
DescriptionReactions (Amounts in Cuvette)
<br.> Kinetics
<br.> Remake BSA-AuNPs
<br.> Reactions: with 1mL HRP-NPs
Data
Notes |