User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/11/12

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==Date==
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==November 12, 2013==
===Objective===
===Objective===
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#Atomic Absorption of nanoparticles
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#UV-vis measurements of Lysozyme and Myoglobin nanoparticles
===Description===
===Description===
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<u>Atomic Absorption:</u><br.>
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*Centrifuge 4mL from each sample for 30min at 4000rpm
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*Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
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*Take measurements of your standards and samples
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*Make a graph showing concentration of Au vs Au:protein ratio
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=====Gold Standards- Calculations=====
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#50 ug/mL:
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#*(1000ug/mL)V=(50ug/mL)(5mL)
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#*V= 0.25mL Au stock in 4.75mL water
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*Each sample diluted from Au stock in the above manner
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===Data===
===Data===

Revision as of 12:53, 12 November 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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November 12, 2013

Objective

  1. Atomic Absorption of nanoparticles
  2. UV-vis measurements of Lysozyme and Myoglobin nanoparticles

Description

Atomic Absorption:

  • Centrifuge 4mL from each sample for 30min at 4000rpm
  • Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
  • Take measurements of your standards and samples
  • Make a graph showing concentration of Au vs Au:protein ratio
Gold Standards- Calculations
  1. 50 ug/mL:
    • (1000ug/mL)V=(50ug/mL)(5mL)
    • V= 0.25mL Au stock in 4.75mL water
  • Each sample diluted from Au stock in the above manner


Data

Notes


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