User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/11/12

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(November 12, 2013)
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===Objective===
===Objective===
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#Atomic Absorption of nanoparticles
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#Atomic Absorption of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013
#UV-vis measurements of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013
#UV-vis measurements of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013
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#*V= 0.25mL Au stock in 4.75mL water
#*V= 0.25mL Au stock in 4.75mL water
*Each sample diluted from Au stock in the above manner
*Each sample diluted from Au stock in the above manner
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===Data===
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<u>Atomic Absorption</u>
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===Data===
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<u>UV-vis Absoprtion</u>
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===Notes===
===Notes===

Revision as of 12:18, 12 November 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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November 12, 2013

Objective

  1. Atomic Absorption of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013
  2. UV-vis measurements of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013

Description

Atomic Absorption:

  • Centrifuge 4mL from each sample for 30min at 4000rpm
  • Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
  • Take measurements of your standards and samples
  • Make a graph showing concentration of Au vs Au:protein ratio
Gold Standards- Calculations
  1. 50 ug/mL:
    • (1000ug/mL)V=(50ug/mL)(5mL)
    • V= 0.25mL Au stock in 4.75mL water
  • Each sample diluted from Au stock in the above manner

Data

Atomic Absorption


UV-vis Absoprtion

Notes


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