User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/18: Difference between revisions

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==February 18, 2014==
==February 18, 2014==
===Objectives===
===Objectives===
*Polydopamine procedure
*Polydopamine procedure for nanofibers
*Myoglobin procedure (repeat)
*Myoglobin procedure for nanofibers (repeat)
===Procedures===
===Procedures===
[Tuesday]
*Preparing scaffolds
*Polydopamine nanofibers
**Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
**Immerse PLA scaffolds in 10mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
***First two scaffolds immersed in solution pH 8.51
**Add (10mM?) protein to solution for 5 hours to allow surface functionalization to occur
***Second two scaffolds immersed in solution pH 8.59
**Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
**Rinse with deionized water and allow scaffold to dry overnight
**Rinse with deionized water and allow scaffold to dry overnight
[Wednesday]
===Observations===
*Polydopamine nanofibers cont'd
*Brute force
**Put scaffold into 10mL test tube to conduct Myoglobin nanofiber procedure
[[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]]  [[Image:Bforce_myo.jpg|300px]]<br.>
*Myoglobin nanofibers
[[Image:Bforce_myo_10x3.jpg|300px]]  [[Image:Bforce_myo3.jpg|300px]]<br.>
**Same as usual, with doubled concentration (0.5mM Au in final solution) making 100:1 and 60:1 ratios again
*Scaffolds after 1 hr in Tris-polydopamine
[[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]]
*Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution




Revision as of 12:09, 18 February 2014

Polydopamine Myoglobin Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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February 18, 2014

Objectives

  • Polydopamine procedure for nanofibers
  • Myoglobin procedure for nanofibers (repeat)

Procedures

  • Preparing scaffolds
    • Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
      • First two scaffolds immersed in solution pH 8.51
      • Second two scaffolds immersed in solution pH 8.59
    • Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
    • Rinse with deionized water and allow scaffold to dry overnight

Observations

  • Brute force

<br.> <br.>

  • Scaffolds after 1 hr in Tris-polydopamine

  • Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution



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