User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 19: Line 19:
===Observations===
===Observations===
*Brute force
*Brute force
 
[[Image:Bforce_myo.jpg|300px]]  [[Image:Bforce_myo_10x3.jpg|300px]]<br.>
[[Image:Bforce_myo3.jpg|300px]]<br.>
*Scaffolds after 1 hr in Tris-polydopamine
*Scaffolds after 1 hr in Tris-polydopamine


Revision as of 11:53, 18 February 2014

Polydopamine Myoglobin Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

February 18, 2014

Objectives

  • Polydopamine procedure for nanofibers
  • Myoglobin procedure for nanofibers (repeat)

Procedures

  • Preparing scaffolds
    • Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
      • First two scaffolds immersed in solution pH 8.51
      • Second two scaffolds immersed in solution pH 8.59
    • Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
    • Rinse with deionized water and allow scaffold to dry overnight

Observations

  • Brute force

<br.> <br.>

  • Scaffolds after 1 hr in Tris-polydopamine
  • Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/02/18&oldid=773939"