User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/01: Difference between revisions
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===Sample Preparation: AgNPs=== | ===Sample Preparation: AgNPs=== | ||
=====Without BSA===== | =====Without BSA===== | ||
*From [http://pubs.acs.org/doi/pdf/10.1021/ed084p322 ] | *From [http://pubs.acs.org/doi/pdf/10.1021/ed084p322 Synthesis and Study of Silver Nanoparticles] | ||
*10 ml of 1 mM AgNO3 was added dropwise (about 1 drop/sec) to 30 ml of 2 mM NaBH4 (cooled in ice bath), while the reaction mixture was vigorously stirred using a magnetic stir plate. Entire procedure takes about 3 minutes. | *10 ml of 1 mM AgNO3 was added dropwise (about 1 drop/sec) to 30 ml of 2 mM NaBH4 (cooled in ice bath), while the reaction mixture was vigorously stirred using a magnetic stir plate. Entire procedure takes about 3 minutes. | ||
=====With BSA (direct from article)===== | =====With BSA (direct from article)===== | ||
*From [http://www.sciencedirect.com/science/article/pii/S0021979712009526 | *From [http://www.sciencedirect.com/science/article/pii/S0021979712009526 Characterization of Silver/Bovine Serum Albumin (Ag/BSA) nanoparticles structure: Morphological, compositional, and interaction studies] | ||
*"Initially 0.9 g of BSA was mixed with 40 ml of deionized water. One milli liter of 0.423 M silver nitrate was added to BSA solution at ∼23 °C and sonicated for 10 min. This was followed by the addition of 1 ml of 0.423 M of sodium borohydride and sonicated for additional 30 min to reduce the silver ions in the solution. The resulting brown homogeneous solution was centrifuged at 21,500 rpm and the residue was washed with ethanol, suspended in water, and saved in an amber colored bottle at 4 °C." | *"Initially 0.9 g of BSA was mixed with 40 ml of deionized water. One milli liter of 0.423 M silver nitrate was added to BSA solution at ∼23 °C and sonicated for 10 min. This was followed by the addition of 1 ml of 0.423 M of sodium borohydride and sonicated for additional 30 min to reduce the silver ions in the solution. The resulting brown homogeneous solution was centrifuged at 21,500 rpm and the residue was washed with ethanol, suspended in water, and saved in an amber colored bottle at 4 °C." | ||
Revision as of 10:37, 1 April 2014
Cell/Scaffold Tests | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
April 1, 2014Objectives
Au:Myoglobin Nanofibers: Sample Prep for Cell Culture
Plain Scaffolds
Brute Force Nanofiber-coated Scaffolds
Original Method for Nanofiber-coated Scaffolds
Polydopamine-coated Scaffolds
Polydopamine Nanofiber-coated Scaffolds
BreakdownToday
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Sample Preparation: AgNPsWithout BSA
With BSA (direct from article)
Type I Collagen Test (Detection and Quantification)Collagen Staining Procedure & Microscope ImagingTrypsin Cell Detachment Procedure & Cell Counting |