User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/01: Difference between revisions
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*Autoclaved | *Autoclaved | ||
===Breakdown | ===Breakdown=== | ||
=====Today=====- | |||
*Time sensitive: Make 40mL TRIS-polydopamine solution | *Time sensitive: Make 40mL TRIS-polydopamine solution | ||
*Time sensitive: Make 6 tests tubes of TRIS-polydopamine solution, put scaffolds in all 6 test tubes at '''12 p.m.''' (3 for pD only, 3 for pD-original) | *Time sensitive: Make 6 tests tubes of TRIS-polydopamine solution, put scaffolds in all 6 test tubes at '''12 p.m.''' (3 for pD only, 3 for pD-original) | ||
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*Time Sensitive: At '''6 p.m.''' rinse pD scaffolds that have soaked for 5 hours, submerge 6 in water overnight (if possible, submerge 3 of 6 tubes in Au:Myo solution and put all 6 in oven for 4 hours), dump Au:Myo fibers onto scaffolds for brute force | *Time Sensitive: At '''6 p.m.''' rinse pD scaffolds that have soaked for 5 hours, submerge 6 in water overnight (if possible, submerge 3 of 6 tubes in Au:Myo solution and put all 6 in oven for 4 hours), dump Au:Myo fibers onto scaffolds for brute force | ||
=== | =====Tomorrow===== | ||
*If oven was not finished yesterday: submerge 3 pD scaffolds into original solution, leave other 3 in water and put in oven for 4 hours at 80C | *If oven was not finished yesterday: submerge 3 pD scaffolds into original solution, leave other 3 in water and put in oven for 4 hours at 80C | ||
*Submerged dried brute force scaffolds in water | *Submerged dried brute force scaffolds in water |
Revision as of 03:54, 1 April 2014
Cell/Scaffold Tests | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
April 1, 2014Objectives
Au:Myoglobin Nanofibers: Sample Prep for Cell Culture
Plain Scaffolds
Brute Force Nanofiber-coated Scaffolds
Original Method for Nanofiber-coated Scaffolds
Polydopamine-coated Scaffolds
Polydopamine Nanofiber-coated Scaffolds
Breakdown=====Today=====-
Tomorrow
Type I Collagen Test (Detection and Quantification)Collagen Staining Procedure & Microscope ImagingTrypsin Cell Detachment Procedure & Cell Counting |