April 1, 2014
Objectives
Au:Myoglobin Nanofibers: Sample Prep for Cell Culture
- Solutions:
- Au: 0.011g in 10mL to make 2.793mM solution
- Myoglobin: 0.011g in 10mL to make 0.0621mM solution
- TRIS dopamine: 0.038g dopamine hydrochloride and 0.024g TRIS in 20mL to make 10mM solution at pH 8.46
Plain Scaffolds
- 4 PLA scaffolds printed for each test tube
- Three test tubes filled with scaffolds and water
- Autoclaved
Brute Force Nanofiber-coated Scaffolds
- 4 PLA scaffolds printed for each test tube
- Gold:Myoglobin (100:1) nanofibers synthesized by above method
- Nanofibers dried onto PLA scaffolds
- Scaffolds submerged in water
- Autoclaved
Original Method for Nanofiber-coated Scaffolds
- 4 PLA scaffolds printed for each test tube
- Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with scaffolds in solution
- Autoclaved
Polydopamine-coated Scaffolds
- 4 PLA scaffolds printed for each test tube
- Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour
- Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
- Rinse with deionized water and allow scaffold to dry overnight
- Scaffolds submerged in water
- Autoclaved
Polydopamine Nanofiber-coated Scaffolds
- 4 PLA scaffolds printed for each test tube
- Scaffolds submerged in 10mM pH 8.5 TRIS dopamine hydrochloride solution for 1 hour
- Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
- Rinse with deionized water and allow scaffold to dry overnight
- Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution
- Autoclaved
Type I Collagen Test (Detection and Quantification)
Collagen Staining Procedure & Microscope Imaging
Trypsin Cell Detachment Procedure & Cell Counting
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Cell/Scaffold Tests
