User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/04/22: Difference between revisions
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===Test Procedures=== | ===Test Procedures=== | ||
====Type I Collagen Test (Detection and Quantification)===== | =====Type I Collagen Test (Detection and Quantification)===== | ||
*[http://www.chondrex.com/products/mouse-type-i-collagen-detection-kit Company procedure ] | *[http://www.chondrex.com/products/mouse-type-i-collagen-detection-kit Company procedure ] | ||
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=====Collagen Staining Procedure & Microscope Imaging Test===== | =====Collagen Staining Procedure & Microscope Imaging Test===== | ||
*Absorbance data: | *Absorbance data: | ||
[[Image:2014_0422_abs_collagen_quantification_test.PNG]] | |||
*Calculations (from company procedure) | *Calculations (from company procedure) | ||
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**Collagen (ug/3 scaffold layers)= [OD 540 value-(OD 605 value x 0.291)]/0.0378 | **Collagen (ug/3 scaffold layers)= [OD 540 value-(OD 605 value x 0.291)]/0.0378 | ||
**Non-collagenous proteins (ug/3 scaffold layers)= OD 605 value/0.00204 | **Non-collagenous proteins (ug/3 scaffold layers)= OD 605 value/0.00204 | ||
[[Image:2014_0422_collagen_quantification_calcs.png]] | |||
=====Trypsin Cell Detachment Procedure & Cell Counting===== | =====Trypsin Cell Detachment Procedure & Cell Counting===== | ||
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===Next time=== | ===Next time=== | ||
*Perform all three tests in triplicate | *Perform all three tests in triplicate | ||
*Have one scaffold for assays that has no cells one it (for blank & with "leftover" media [under microscope?]) | |||
*Keep solutions post-assay to look at under microscope to see if cells are still there | |||
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Revision as of 11:57, 23 April 2014
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April 22, 2014Objectives
Test ProceduresType I Collagen Test (Detection and Quantification)Collagen Staining Procedure & Microscope ImagingTrypsin Cell Detachment Procedure & Cell CountingTest ResultsCollagen Staining Procedure & Microscope Imaging Test
Trypsin Cell Detachment Procedure & Cell CountingPerformed on 3 scaffold layers.
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