User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/09/26: Difference between revisions
From OpenWetWare
(Autocreate 2014/09/26 Entry for User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S) |
(fix raw html notebook nav) |
||
(5 intermediate revisions by one other user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Nanofiber Coated Scaffolds</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==September 26, 2014== | ||
* | ===Procedures=== | ||
====Solution Prep==== | |||
*Au stock solution: | |||
**0.011g x (1 mol/393.833g) x 0.010L = 2.793mM | |||
*Myoglobin stock solution: | |||
**0.011g x (1 mol/17699g) x 0.010L = 0.0621mM | |||
====Nanofiber Synthesis==== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Protein''' | |||
| align="center" style="background:#f0f0f0;"|'''Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Final [Au]''' | |||
| align="center" style="background:#f0f0f0;"|'''Vol Au''' | |||
| align="center" style="background:#f0f0f0;"|'''Vol Protein''' | |||
| align="center" style="background:#f0f0f0;"|'''Vol Water''' | |||
|- | |||
| Myoglobin|| 100:1|| 0.5 mM || 0.895 mL|| 0.403 mL|| 3.702 mL | |||
|- | |||
| || || 0.25 mM|| 0.448 mL|| 0.201 mL|| 4.351 mL | |||
|} | |||
*Measure solutions into 10 mL glass test tubes | |||
**8 solutions total | |||
*Stack 4 of the same type of scaffold into each tube | |||
*Heat at 80C for 4 hours | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 00:21, 27 September 2017
Nanofiber Coated Scaffolds | Main project page Previous entry Next entry | ||||||||||||||||||
September 26, 2014ProceduresSolution Prep
Nanofiber Synthesis
|