User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/09/26: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 27: Line 27:
| || || 0.25 mM|| 0.448 mL|| 0.201 mL|| 4.351 mL
| || || 0.25 mM|| 0.448 mL|| 0.201 mL|| 4.351 mL
|}
|}
*Measure solutions into 10 mL glass test tubes
**8 solutions total
*Stack 4 of the same type of scaffold into each tube
*Heat at 80C for 4 hours


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 12:02, 24 September 2014

Nanofiber Coated Scaffolds <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

September 26, 2014

Procedures

Solution Prep

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM

Nanofiber Synthesis

Protein Ratio Final [Au] Vol Au Vol Protein Vol Water
Myoglobin 100:1 0.5 mM 0.895 mL 0.403 mL 3.702 mL
0.25 mM 0.448 mL 0.201 mL 4.351 mL
  • Measure solutions into 10 mL glass test tubes
    • 8 solutions total
  • Stack 4 of the same type of scaffold into each tube
  • Heat at 80C for 4 hours


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/CHEM-572_2014S/2014/09/26&oldid=823315"