User:Madeleine Y. Bee/Notebook/CHEM-581 Experimental Chemistry/2014/09/05: Difference between revisions

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===Procedures===
===Procedures===
====Completing Film Synthesis====
====Completing Film Synthesis====
Continued from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-581_Experimental_Chemistry/2014/08/27 Friday, 8/29/2014]<br.>
Continued from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-581_Experimental_Chemistry/2014/09/03 Wednesday, 9/3/2014]<br.>
Procedure detailed by [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/08/29 Dr Hartings]
Procedure detailed by [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/08/29 Dr Hartings]
*Stir in Sodium Sulfate, 1% HCl, and Sodium Bicarbonate solutions
*Stir in Sodium Sulfate, 1% HCl, and Sodium Bicarbonate solutions

Revision as of 12:52, 8 September 2014

Standard and Film Soln Absorbance <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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September 5, 2014

Procedures

Completing Film Synthesis

Continued from Wednesday, 9/3/2014<br.> Procedure detailed by Dr Hartings

  • Stir in Sodium Sulfate, 1% HCl, and Sodium Bicarbonate solutions

Beginning New Film Synthesis

Procedure detailed by Dr Hartings

  • Polymer-10% Clay Film
    • 1.0004 g PVA and 0/1077 g sodium montmorillonite (~1g PVA and ~.1g NaM)
  • Polymer Film
    • 1.0050 g PVA (~1g)

UV-vis Measurements

  • Take UV Vis spectra for Malachite green at the following concentrations: 0ppm, 0.20ppm, 0.50ppm, 1.1ppm, 1.4ppm, 1.7ppm, and 2.0ppm
  • Construct calibration curve for each of the two absorbance maximums

<br.>

Equilibrium Measurements

  • Dilute 200 ppm solutions 100x and 80 ppm solutions 50x to 10 mL & transfer to glass test tube (3 mL needed for absorbance measurements)

  • Determine the amount of Malachite green absorbed into a film
    • ε615=0.1293<br.>
    • A615=ε615bc → c=A615/ε615b:<br.>
    1. PVA film in 200 ppm
      • csoln=(0.092)/(0.1293)(1)= 0.716M= ppm ×100=
    2. PVA film in 80 ppm
      • csoln=(0.094)/(0.1293)(1)= 0.727M= ppm ×50=
    3. PVA film in 8 ppm
      • csoln=(0.064)/(0.1293)(1)= 0.495M= ppm
    4. PVA film in 2 ppm
      • csoln=(0.009)/(0.1293)(1)= 0.070M= ppm
    5. PVA Clay film in 200 ppm
      • csoln=(0.019)/(0.1293)(1)= 0.147M= ppm ×100=
    6. PVA Clay film in 80 ppm
      • csoln=(0.010)/(0.1293)(1)= 0.077M= ppm ×50=
    7. PVA Clay film in 8 ppm
      • csoln=(0.041)/(0.1293)(1)= 0.317M= ppm
    8. PVA Clay film in 2 ppm
      • csoln=(0.004)/(0.1293)(1)= 0.031M= ppm
    • ε425=0.0266<br.>
    • A425=ε425bc → c=A425/ε425b:<br.>
    1. PVA film in 200 ppm
      • csoln=(0.019)/(0.1293)(1)= 0.147M= ppm ×100=
    2. PVA film in 80 ppm
      • csoln=(0.020)/(0.1293)(1)= 0.155M= ppm ×50=
    3. PVA film in 8 ppm
      • csoln=(0.017)/(0.1293)(1)= 0.131M= ppm
    4. PVA film in 2 ppm
      • csoln=(0.003)/(0.1293)(1)= 0.023M= ppm
    5. PVA Clay film in 200 ppm
      • csoln=(0.005)/(0.1293)(1)= 0.039M= ppm ×100=
    6. PVA Clay film in 80 ppm
      • csoln=(0.003)/(0.1293)(1)= 0.023M= ppm ×50=
    7. PVA Clay film in 8 ppm
      • csoln=(0.006)/(0.1293)(1)= 0.046M= ppm
    8. PVA Clay film in 2 ppm
      • csoln=(0.003)/(0.1293)(1)= 0.023M= ppm
  • Determine the equilibrium of free Malachite green vs bound malachite green with I.C.E. table
  • Determine the mass of malachite green absorbed per mass of film



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