User:Madeleine Y. Bee/Notebook/CHEM-581 Experimental Chemistry/2014/09/05: Difference between revisions

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{| style="width:75%; height:200px" border="1"
{| style="width:75%; height:200px" border="1"
|-  
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| Compound || Malachite Green || NaMT
| || Malachite Green  
|-  
|-  
| Initial Concentration || mno || pqr
| Initial Concentration || mno
|-
|-
| Change in Concentration || vwx || yz
| Change in Concentration || vwx
|-
|-
| Equilibrium Concentration ||    ||
| Equilibrium Concentration ||
|}
|}
*Determine the mass of malachite green absorbed per mass of film
*Determine the mass of malachite green absorbed per mass of film

Revision as of 17:17, 9 September 2014

Standard and Film Soln Absorbance <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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September 5, 2014

Procedures

Completing Film Synthesis

Continued from Wednesday, 9/3/2014<br.> Procedure detailed by Dr Hartings

  • Stir in Sodium Sulfate, 1% HCl, and Sodium Bicarbonate solutions

Beginning New Film Synthesis

Procedure detailed by Dr Hartings

  • Polymer-10% Clay Film
    • 1.0004 g PVA and 0/1077 g sodium montmorillonite (~1g PVA and ~.1g NaM)
  • Polymer Film
    • 1.0050 g PVA (~1g)

UV-vis Measurements

  • Take UV Vis spectra for Malachite green at the following concentrations: 0ppm, 0.20ppm, 0.50ppm, 1.1ppm, 1.4ppm, 1.7ppm, and 2.0ppm
  • Construct calibration curve for each of the two absorbance maximums

<br.>

Equilibrium Measurements

  • Dilute 200 ppm solutions 100x and 80 ppm solutions 50x to 10 mL & transfer to glass test tube (3 mL needed for absorbance measurements)

  • Determine the amount of Malachite green absorbed into a film
    • ε615=0.1293<br.>
    • A615=ε615bc → c=A615/ε615b:<br.>
    1. PVA film in 200 ppm
      • csoln=(0.092)/(0.1293)(1)= 0.716 ppm ×100= 71.6 ppm
    2. PVA film in 80 ppm
      • csoln=(0.094)/(0.1293)(1)= 0.727 ppm ×50= 36.35 ppm
    3. PVA film in 8 ppm
      • csoln=(0.064)/(0.1293)(1)= 0.495 ppm
    4. PVA film in 2 ppm
      • csoln=(0.009)/(0.1293)(1)= 0.070 ppm
    5. PVA Clay film in 200 ppm
      • csoln=(0.019)/(0.1293)(1)= 0.147 ppm ×100= 14.7 ppm
    6. PVA Clay film in 80 ppm
      • csoln=(0.010)/(0.1293)(1)= 0.077ppm ×50= 3.85 ppm
    7. PVA Clay film in 8 ppm
      • csoln=(0.041)/(0.1293)(1)= 0.317 ppm
    8. PVA Clay film in 2 ppm
      • csoln=(0.004)/(0.1293)(1)= 0.031 ppm
    • ε425=0.0266<br.>
    • A425=ε425bc → c=A425/ε425b:<br.>
    1. PVA film in 200 ppm
      • csoln=(0.019)/(0.0266)(1)= 0.714 ppm ×100= 71.4 ppm
    2. PVA film in 80 ppm
      • csoln=(0.020)/(0.0266)(1)= 0.752 ppm ×50= 37.6 ppm
    3. PVA film in 8 ppm
      • csoln=(0.017)/(0.0266)(1)= 0.639 ppm
    4. PVA film in 2 ppm
      • csoln=(0.003)/(0.0266)(1)= 0.113 ppm
    5. PVA Clay film in 200 ppm
      • csoln=(0.005)/(0.0266)(1)= 0.188 ppm ×100= 18.8 ppm
    6. PVA Clay film in 80 ppm
      • csoln=(0.003)/(0.0266)(1)= 0.113 ppm ×50= 5.65 ppm
    7. PVA Clay film in 8 ppm
      • csoln=(0.006)/(0.0266)(1)= 0.226 ppm
    8. PVA Clay film in 2 ppm
      • csoln=(0.003)/(0.0266)(1)= 0.113 ppm
  • Determine the equilibrium of free Malachite green vs bound malachite green with I.C.E. table
Malachite Green
Initial Concentration mno
Change in Concentration vwx
Equilibrium Concentration
  • Determine the mass of malachite green absorbed per mass of film



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